Changes in serum proteins, hematology and liver histology in water depriv

Date : Sep 2008 - Jul 2009

Water deprivation or dehydration has been increasingly seen in all over the world. Dehydration means a state of total body water deficit due to primary water losses or to inadequate water intake. As a common trend, people prefer to take beverages instead of plain water. They contain caffeine, alcohol, sugar, artificial sweeteners or other chemicals that act as strong dehydrators. An experiment was conducted to assess the effects of various duration of water deprivation on blood protein profile, hematology and liver histology in rat models. Gel electrophoresis and Syngene genius system were used to study the protein profile of serum. Hematology and liver histology were also studied. In the analysis of higher molecular weight proteins a gradual decrease in % raw volume was observed in the proteins corresponding to the molecular weights of 116, 97, 66 kDa. Increase in concentration in lower molecular weight serum proteins corresponding to the molecular weight 24, 20 and 14.2 kDa was observed. Statistical analysis by one way ANOVA during the whole study determined a significant increase in hematocrit (P=0.0038**), hemoglobin (P=0.0009***) and RBCs (P=0.0040**). While decrease in WBCs was non-significant (P=0.0666) statistically. Fluctuation in Mean corpuscular hemoglobin (P=0.5217), Mean corpuscular hemoglobin concentration (P=0.1211), Mean corpuscular volume (P=0.7147), Platelets (P=0.0653), Mean platelets volume (P=0.3906) and Chloride (P=0.5273) were found non-significant statistically. Liver histology was studied and after three days of water deprivation an enlargement of the nuclei and more pronounced intracellular spaces was observed. Another common alteration was the dilation in the blood vessels and recruitment of some cells towards the blood vessels on 3rd and 6th day of experiment. On 9th day a destruction of tissue was more prevalent over all other alterations.